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KMID : 1059519900340060622
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Journal of the Korean Chemical Society
1990 Volume.34 No. 6 p.622 ~ p.628
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Determination of Glutamine Utilizing New Plant Tissue Bio-Sensor
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Ihn Gwon-Shik
Kim Bong-Weon
Jeon Young-Guk
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Abstract
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The bio-sensor for glutamine has been constructed by immobilizing petal of the rose structural elements on an ammonia gas sensor. This sensor was investigated for the effects of pH, temperature, buffer solution, tissular amounts, interferences and lifetime. As a result, the tissue sensor showed linear range of 8.0 × 10-4 - 5.0 × 10-2 M glutamine with a slope of 52 mV/decade in pH 7.8, 0.2M phosphate beffer solution at 37℃. The tissular amounts used for this sensor was 50 mg. This sensorr showed excellent selectivity. This sensor was compared with other structural elements of rose. Actually, this tissue sensor appeared to be very useful for the determination of glutamine.
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KEYWORD
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